Transmission-blocking antibodies against mosquito C-type lectins for dengue prevention.

Transmission-blocking antibodies against mosquito C-type lectins for dengue prevention.

Blog Article

C-type lectins are a family of proteins with carbohydrate-binding activity.Several C-type lectins in mammals or arthropods are employed as receptors or attachment factors to facilitate flavivirus invasion.We previously identified a C-type lectin in Aedes aegypti, designated as mosquito galactose specific C-type lectin-1 (mosGCTL-1), facilitating the 15-eg2373cl attachment of West Nile virus (WNV) on the cell membrane.Here, we first identified that 9 A.aegypti mosGCTL genes were key susceptibility factors facilitating DENV-2 infection, of which mosGCTL-3 exhibited the most significant effect.

We found that mosGCTL-3 was induced in mosquito tissues with DENV-2 infection, and that the protein interacted with DENV-2 surface envelop (E) protein and virions in vitro and in vivo.In addition, the other identified mosGCTLs interacted with the DENV-2 E protein, indicating that DENV may employ multiple mosGCTLs as ligands to promote the infection of vectors.The vectorial susceptibility factors that facilitate pathogen invasion may potentially be explored as a target to disrupt the acquisition of microbes from the vertebrate host.Indeed, membrane blood feeding opheliasmuse.com of antisera against mosGCTLs dramatically reduced mosquito infective ratio.Hence, the immunization against mosGCTLs is a feasible approach for preventing dengue infection.

Our study provides a future avenue for developing a transmission-blocking vaccine that interrupts the life cycle of dengue virus and reduces disease burden.